Journal
FRONTIERS IN CHEMISTRY
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2019.00130
Keywords
imprinted array film; triazophos; CdSe/ZnS QDs probe; fluorescence assay; cabbage and apple
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Funding
- National Natural Science Foundation of China [31471654, 31772071, 31501571]
- Guangxi Innovation-driven Development Project [AA17204043-2]
- China Agriculture Research System [CARS-05-05A-03]
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In the present study we have developed a direct competitive CdSe/ZnS quantumdot (QD) fluorescence assay based on micro-array-imprinted membranes for the determination of triazophos in cabbage and apple. The imprinted membranes were directly synthesized on the surface of a 96-well plate by thermal polymerization using triadimefon as the dummy template. Under optimal conditions, the assay showed an excellent linear response over the concentration ranges of 0.1-10,000 mu g L-1 with a good coefficient of determination (R-2= 0.982). The sensitivity (IC50) and limit of detection (LOD, expressed as IC15) of the developed assay were 3.63 mg L-1 and 0.31 mu g L-1, respectively. The applicability of the developed approach was tested for detecting triazophos in incurred samples. Themethod showed excellent recoveries (109.6-118.9%) and relative standard deviations (RSDs) between 9.9 and 19.5%. The obtained results correlated well with those obtained by LC-MS/MS (R-2= 0.9995). The competitive assay using CdSe/ZnS QDs as fluorescence-labeled probe showed good sensitivity, steady and fast response, and excellent anti-interference ability compared to conventional fluorescence-quenching methods. Finally, the feasibility of the proposed methodology was successfully applied for detection of triazophos in real samples.
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