Journal
ANIMAL REPRODUCTION SCIENCE
Volume 172, Issue -, Pages 143-147Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.anireprosci.2016.07.012
Keywords
Artificial fertilization; Embryo survival; Cyprinids; Burbot
Funding
- Innovations in finfish aquaculture with special references to reproduction, Operational Programme Sustainable Development of the Fisheries Sector and Coastal Fishing Areas [OR14-61724-OR1400003/09/10/11]
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The development of a new protocol for egg fertilization may increase embryo survival and benefit the aquaculture process. In the present study, a new technique of partially adding sperm to activated eggs in the artificial fertilization of burbot (Lota lota), ide (Leuciscus idus) and asp (Aspius aspius) eggs was evaluated. If the same volume of sperm was divided into two or three parts and added to eggs in 30-60 s intervals, it significantly improved embryo survival at the eyed-egg-stage of development. In the present study, the periodic addition of spermatozoa to eggs affected fertilization (ide and asp) and embryo survival rates (ide, asp and burbot) and might be successfully applied under hatchery conditions. (C) 2016 Elsevier B.V. All rights reserved.
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