Journal
MYCOBIOLOGY
Volume 47, Issue 1, Pages 76-86Publisher
KOREAN SOC MYCOLOGY
DOI: 10.1080/12298093.2019.1572263
Keywords
Genetic diversity; pear; scab; Venturia nashicola
Funding
- Cooperative Research Program for Agricultural Science and Technology Development [PJ01019702, PJ011879]
- Rural Development Administration, Republic of Korea
Ask authors/readers for more resources
Scab disease caused by Venturia nashicola is of agroeconomic importance in cultivation of Asian pear. However, little is known about the degree of genetic diversity in the populations of this pathogen. In this study, we collected 55 isolates from pear scab lesions in 13 major cultivation areas in Korea and examined the diversity using sequences of internal transcribed spacer (ITS) region, beta-tubulin (TUB2), and translation elongation factor-1 alpha (TEF-1 alpha) genes as molecular markers. Despite a low level of overall sequence variation, we found three distinctive subgroups from phylogenetic analysis of combined ITS, TUB2, and TEF-1 alpha sequences. Among the three subgroups, subgroup 1 (60% of isolates collected) was predominant compared to subgroup 2 (23.6%) or subgroup 3 (16.4%) and was distributed throughout Korea. To understand the genetic diversity among the subgroups, RAPD analysis was performed. The isolates yielded highly diverse amplicon patterns and none of the defined subgroups within the dendrogram were supported by bootstrap values greater than 30%. Moreover, there is no significant correlation between the geographical distribution and the subgroups defined by molecular phylogeny. Our data suggest a low level of genetic diversification among the populations of V. nashicola in Korea.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available