Journal
JOURNAL OF SYNCHROTRON RADIATION
Volume 26, Issue -, Pages 406-412Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600577519000304
Keywords
serial synchrotron crystallography; microfluidics; flow focusing
Categories
Funding
- Deutsche Forschungsgemeinschaft [Exzellenzcluster 1074]
- Bundesministerium fur Bildung und Forschung [05K16GUI]
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Serial synchrotron crystallography allows low X-ray dose, room-temperature crystal structures of proteins to be determined from a population of microcrystals. Protein production and crystallization is a non-trivial procedure and it is essential to have X-ray-compatible sample environments that keep sample consumption low and the crystals in their native environment. This article presents a fast and optimized manufacturing route to metal-polyimide microfluidic flow-focusing devices which allow for the collection of X-ray diffraction data in flow. The flow-focusing conditions allow for sample consumption to be significantly decreased, while also opening up the possibility of more complex experiments such as rapid mixing for time-resolved serial crystallography. This high-repetition-rate experiment allows for full datasets to be obtained quickly (approximate to 1h) from crystal slurries in liquid flow. The X-ray compatible microfluidic chips are easily manufacturable, reliable and durable and require sample-flow rates on the order of only 30 mu lh(-1).
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