Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 5, Issue 5, Pages 2647-2656Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.8b01605
Keywords
CTC chamber; cell-replicated surface; PDMS; capture; cancer cells
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Funding
- National Natural Science Foundation of China [51773026]
- Fundamental Research Fund for the National Key Technologies RD Program [2016YFC1103002]
- Fundamental Research Fund for the Central Universities [DUT17-LAB06]
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The capture of circulating tumor cells (CTCs) is mainly carried out with a small volume of blood using magnetic nanoparticles and complex microfluidics. In this study, we propose a CTC-capture apparatus based on a modular design and called this apparatus as the CTC chamber. Distinct from other CTC-capture apparatuses, the capacity of the CTC chamber could be altered by varying the number of CTC-capture modules to accommodate the different volumes of blood sample. The core component of the CTC-capture module was a polydimethylsiloxane (PDMS) film with cell-replicated topological structure and anti-EpCAM antibody coating. Both synergistic roles can enhance the capture yield of cancer cells. Furthermore, the CTC chamber was assembled with one or three CTC-capture modules for the capture of cancer cells from spiked blood samples representing late-stage (3 mL of blood, 10 cancer cells mL(-1)) or middle-early stage (9 mL of blood, 1 cancer cell mL(-1)) cancer. The results showed that high capture yield (EpCAM-positive, similar to 80%; EpCAM-negative, similar to 65%) and purity (EpCAM-positive, similar to 90%; EpCAM-negative, similar to 80%) could be obtained within 1 h. This economic and facile CTC chamber could therefore open up opportunities for designing the next-generation CTC detection devices suitable for the diagnosis of different stages of cancer.
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