Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 5, Issue 3, Pages 1189-1194Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.8b01054
Keywords
mucus; mucin; antibiotics; efficacy; Pseudomonas aeruginosa
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Funding
- National Institutes of Health [NIH R01-EB017755]
- National Science Foundation [PHY-1454673]
- MRSEC Program of the National Science Foundation [DMR-14-19807]
- National Science Foundation Graduate Research Fellowship [1122374]
- Siebel Scholarship
- MIT Collamore-Rogers Fellowship
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Mucus, a biopolymer hydrogel that covers all wet epithelia of the body, is a potential site for infection by pathogenic bacteria. Mucus can bind small molecules and influence bacterial physiology, two factors that may affect the efficacy of antibiotics. In spite of this, the impact of mucus on antibiotic activity has not been thoroughly characterized. We examined the activity of polymyxin and fluoroquinolone antibiotics against the opportunistic pathogen Pseudomonas aeruginosa in native mucus and purified mucin biopolymer environments. We found that mucus reduces the effectiveness of polymyxins and fluoroquinolones against P. aeruginosa. Mucin biopolymers MUCSAC, MUC2, and MUCSB are primary contributors to this reduction. Our findings highlight that the biomaterial environmental context should be considered when evaluating antibiotics in vitro.
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