Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 5, Issue 3, Pages 1392-1404Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.8b01179
Keywords
magnetic nanoparticles; magnetic alignment; anisotropic hydrogels; ordered tissues
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Funding
- European Union [706996, 772817]
- Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BPD/112459/2015, UTAP-ICDT/CTM-BIO/0023/2014, PTDC/CTM-BIO/4706/2014 - POCI-01-0145-FEDER 016716, NORTE-01-0145-FEDER-000021]
- Marie Curie Actions (MSCA) [706996] Funding Source: Marie Curie Actions (MSCA)
- Fundação para a Ciência e a Tecnologia [UTAP-ICDT/CTM-BIO/0023/2014] Funding Source: FCT
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Injectable hydrogels are particularly interesting for applications in minimally invasive tissue engineering and regenerative medicine strategies. However, the typical isotropic microstructure of these biomaterials limits their potential for the regeneration of ordered tissues. In the present work, we decorated rod-shaped cellulose nanocrystals with magnetic nanoparticles and coated these with polydopamine and polyethylene glycol polymer brushes to obtain chemical and colloidal stable nanoparticles. Then, these nanoparticles (0.1-0.5 wt %) were incorporated within gelatin hydrogels, creating injectable and magnetically responsive materials with potential for various biomedical applications. Nanoparticle alignment within the hydrogel matrix was achieved under exposure to uniform low magnetic fields (108 mT), resulting in biomaterials with directional microstructure and anisotropic mechanical properties. The biological performance of these nanocomposite hydrogels was studied using adipose tissue derived human stem cells. Cells encapsulated in the nanocomposite hydrogels showed high rates of viability demonstrating that the nanocomposite biomaterials are not cytotoxic. Remarkably, the microstructural patterns stemming from nanoparticle alignment induced the directional growth of seeded and, to a lower extent, encapsulated cells in the hydrogels, suggesting that this injectable system might find application in both cellular and acellular strategies targeting the regeneration of anisotropic tissues.
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