Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 143, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/59127
Keywords
Bioengineering; Issue 143; Electrical stimulation chamber; cell culture; MSC; osteogenic differentiation; direct current; 6-well plate
Categories
Funding
- AO Foundation Start-Up Grant [S-14-03H]
- Friedrichsheim Foundation (Stiftung Friedrichsheim) based in Frankfurt/Main, Germany
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Mesenchymal stem/stromal cells (MSCs) have been used extensively to promote bone healing in tissue engineering approaches. Electrical stimulation (EStim) has been demonstrated to increase MSC osteogenic differentiation in vitro and promote bone healing in clinical settings. Here we describe the construction of an EStim cell culture chamber and its use in treating rat bone-marrow-derived MSC to enhance osteogenic differentiation. We found that treating MSCs with EStim for 7 days results in a significant increase in the osteogenic differentiation, and importantly, this pro-osteogenic effect persists long after (7 days) EStim is discontinued. This approach of pretreating MSCs with EStim to enhance osteogenic differentiation could be used to optimize bone tissue engineering treatment outcomes and, thus, help them to achieve their full therapeutic potential. In addition to this application, this EStim cell culture chamber and protocol can also be used to investigate other EStim-sensitive cell behaviors, such as migration, proliferation, apoptosis, and scaffold attachment.
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