Journal
ANIMAL CELLS AND SYSTEMS
Volume 20, Issue 2, Pages 63-69Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/19768354.2016.1165288
Keywords
MicroRNA; mammary epithelial cell; flow cytometry; hoechst33342/PI
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Funding
- Shandong Provincial Natural Science Foundation [ZR2014CM029]
- Shandong Province Higher Educational Science and Technology Programme [J14LF07]
- Key R & D projects in Shandong Province [2015GNC110003]
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MicroRNAs are a class of post-transcriptional regulators of gene expression in multicellular organisms, which play important roles in cell fate, organ morphogenesis and carcinogenesis. In the present study, we demonstrated the critical roles of miR-143 on mammary epithelial cells of dairy goat. The test results of cell count, methylthiazolyldiphenyl-tetrazolium bromide, Hoechst33342/PI (propidium iodide) and flow cytometry showed miR-143-induced G0/G1 phase arrest, inhibited proliferation and promoted apoptosis of mammary epithelial cells; the qRT-PCR test of marker genes related to cell proliferation and apoptosis, BAX and BCL-2, supported the same conclusions. Our study presents evidence that miR-143 is an important post-transcription regulator involving in mammary cells survival, and it may have a value function in mammary gland development, lactation or involution.
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