Journal
GENES
Volume 10, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/genes10030196
Keywords
replication timing; cohesin; genome architecture; chromatin interactions; DNA
Categories
Funding
- Biotechnology and Biological Sciences Research Council [BB/N016858/1]
- Wellcome Trust [203727/Z/16/Z, 110064/Z/15/Z]
- BBSRC [BB/N016858/1] Funding Source: UKRI
- Wellcome Trust [110064/Z/15/Z, 203727/Z/16/Z] Funding Source: Wellcome Trust
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3D genome organization is strongly predictive of DNA replication timing in mammalian cells. This work tested the extent to which loop-based genome architecture acts as a regulatory unit of replication timing by using an auxin-inducible system for acute cohesin ablation. Cohesin ablation in a population of cells in asynchronous culture was shown not to disrupt patterns of replication timing as assayed by replication sequencing (RepliSeq) or BrdU-focus microscopy. Furthermore, cohesin ablation prior to S phase entry in synchronized cells was similarly shown to not impact replication timing patterns. These results suggest that cohesin-mediated genome architecture is not required for the execution of replication timing patterns in S phase, nor for the establishment of replication timing domains in G1.
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