Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 10, Pages 3442-3446Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201511640
Keywords
catenanes; protein structures; protein folding; supramolecular chemistry; topology
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Funding
- 863 Program [2015AA020941]
- National Natural Science Foundation of China [21474003, 91427304]
- 1000 Plan (Youth)
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Direct cellular production of topologically complex proteins is of great interest both in supramolecular chemistry and protein engineering. We describe the first cellular synthesis of protein catenanes through the use of the p53 dimerization domain to guide the intertwining of two protein chains and SpyTag-SpyCatcher chemistry for efficient cyclization. The catenane topology was unambiguously proven by SDS-PAGE, SEC, and partial digestion experiments and was shown to confer enhanced stability toward trypsin digestion relative to monomeric control mutants. The assembly-reaction synergy enabled by protein folding and genetically encoded protein chemistry offers a convenient yet powerful approach for creating mechanically interlocked, complex protein topologies invivo.
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