4.7 Article

Genome Survey Sequencing of Acer truncatum Bunge to Identify Genomic Information, Simple Sequence Repeat (SSR) Markers and Complete Chloroplast Genome

Journal

FORESTS
Volume 10, Issue 2, Pages -

Publisher

MDPI
DOI: 10.3390/f10020087

Keywords

Acer truncatum; genome survey; SSR markers; chloroplast genome

Categories

Funding

  1. Key Research and Development Program of Shaanxi Province [2018ZDXM2-01]
  2. Industry Technology Innovation Key Technology Research and Demonstration Project of Acer truncatum Bunge [ZYZB2018-DY1170]
  3. Yangling Demonstration Zone Science and Technology Program [2018NY-26]
  4. Young Talent fund of University Association for Science and Technology in Shaanxi, China [20160107]

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The Acer truncatum Bunge is a particular forest tree species found in the north of China. Due to the recent discovery that its seeds contain a considerable amount of nervonic acid, this species has received more and more attention. However, there have been no reports of the genome in this species. In this study, we report on the Acer truncatum genome sequence produced by genome survey sequencing. In total, we obtained 61.90 Gbp of high-quality data, representing approximately 116x coverage of the Acer truncatum genome. The genomic characteristics of Acer truncatum include a genome size of 529.88 Mbp, a heterozygosis rate of 1.06% and a repeat rate of 48.8%. A total of 392,961 high-quality genomic SSR markers were developed and a graphical map of the annotated circular chloroplast genome was generated. Thus far, this is the first report of de novo whole genome sequencing and assembly of Acer truncatum. We believe that this genome sequence dataset may provide a new resource for future genomic analysis and molecular breeding studies of Acer truncatum.

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