Journal
CHEMPLUSCHEM
Volume 84, Issue 4, Pages 323-327Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cplu.201900025
Keywords
artificial receptors; cell membranes; chemical anchoring; DNA nanostructures; transmembrane transport
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Funding
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support [20171913]
- Shuguang Program - Shanghai Education Development Foundation
- Shanghai Municipal Education Commission [18SG16]
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Highly efficient transmembrane transport of exogenous reagents into cells is of vital importance for developing drug-delivery systems. Conventionally, transmembrane transport of exogenous reagents was accomplished with the assistance of transfection agents or other artificial means. However, the high toxicity and low transport efficiency of current delivery techniques still remain to be solved. In this work, by anchoring artificial receptors onto cell membranes with a mild chemical reaction, we demonstrated that the exogenous reagent framework nucleic acids, namely tetrahedral DNA nanostructures (TDN) can bind onto cell membranes effectively by the hybridization between single-stranded DNA (ssDNA) and pendant ssDNA of TDN. The transport rate was greatly enhanced, with the endocytosis time could be as fast as 0.5h. Furthermore, the transport quantity was prominently improved, with around 30% of TDN endocytosed within 4h. Owing to its rapid transmembrane transport speed and improved endocytosis quantity, this artificial-receptor-mediated transmembrane transport is a promising tool for achieving rapid and highly efficient transmembrane transport of exogenous reagents.
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