Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 28, Pages 8045-8048Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201600177
Keywords
Escherichia coli; [FeFe]-hydrogenase; hydrogen; photocatalysis; whole-cell reactions
Categories
Funding
- International Institute for Carbon-Neutral Energy Research (WPI-I2CNER)
- World Premier International Research Center Initiative (WPI), MEXT, Japan
Ask authors/readers for more resources
A photocatalytic H-2 production system using an inorganic-bio hybrid photocatalyst could contribute to the efficient utilization of solar energy, but would require the development of a new approach for preparing a H-2-forming biocatalyst. In the present study, we constructed a recombinant strain of Escherichia coli expressing the genes encoding the [FeFe]-hydrogenase and relevant maturases from Clostridium acetobutylicum NBRC 13948 for use as a biocatalyst. We investigated the direct application of a whole-cell of the recombinant E. coli. The combination of TiO2, methylviologen, and the recombinant E. coli formed H-2 under light irradiation, demonstrating that whole cells of the recombinant E. coli could be employed for photocatalytic H-2 production without any time-consuming and costly manipulations (for example, enzyme purification). This is the first report of the direct application of a whole-cell reaction of recombinant E. coli to photocatalytic H-2 production.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available