Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 38, Pages 11447-11451Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201603155
Keywords
bioorthogonal chemistry; cell growth; growth factors; titanium; tyrosinase
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Funding
- Japan Society for the Promotion of Science KAKENHI [15H01810, 22220009]
- MOST-RIKEN Joint Research Project
- CAS-JSPS [GJHZ1519]
- Grants-in-Aid for Scientific Research [16K12902] Funding Source: KAKEN
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The generation of metal surfaces with biological properties, such as cell-growth-enhancing and differentiation-inducing abilities, could be potentially exciting for the development of functional materials for use in humans, including artificial dental implants and joint replacements. However, currently the immobilization of proteins on the surfaces of the metals are limited. In this study, we have used a mussel-inspired bioorthogonal approach to design a 3,4-hydroxyphenalyalanine-containing recombinant insulin-like growth-factor-1 using a combination of recombinant DNA technology and tyrosinase treatment for the surface modification of titanium. The modified growth factor prepared in this study exhibited strong binding affinity to titanium, and significantly enhanced the growth of NIH3T3 cells on the surface of titanium.
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