Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 43, Pages 13538-13543Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201605905
Keywords
dyes; FRET; microcapsules; photodynamic therapy; photosensitizers
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Funding
- National Nature Science Foundation of China [21273055, 21433010, 21320102004, 21321063, 21003074]
- National Basic Research Program of China [2013CB932800]
- National Key Foundation for Exploring Scientific Instrument [2013YQ16055108]
- Natural Science Foundation of Jiangsu Province [BK20131407]
- open project of the CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety [NSKF201608]
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Microcapsules obtained by layer-by-layer assembly provide a good platform for biological analysis owing to their component diversity, multiple binding sites, and controllable wall thickness. Herein, different assembly species were obtained from two-photon dyes and traditional photosensitizers, and further assembled into microcapsules. Fluorescence resonance energy transfer (FRET) was shown to occur between the two-photon dyes and photosensitizers. Confocal laser scanning microscopy (CLSM) with one- and two-photon lasers, fluorescence lifetime imaging microscopy (FLIM), and time-resolved fluorescence spectroscopy were used to analyze the FRET effects in the microcapsules. The FRET efficiency could easily be controlled through changing the assembly sequence. Furthermore, the capsules are phototoxic upon one- or two-photon excitation. These materials are thus expected to be applicable in two-photon-activated photodynamic therapy for deep-tissue treatment.
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