Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 18, Pages 5394-5399Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201511441
Keywords
Cas9; conditional control; CRISPR; genomic editing; light; small molecules
Categories
Funding
- National Science Foundation [MCB-1330746, CHE-1404836]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1404836] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1330746] Funding Source: National Science Foundation
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The recently discovered CRISPR/Cas9 endonuclease system, comprised of a guide RNA for the recognition of a DNA target and the Cas9 nuclease protein for binding and processing the target, has been extensively studied and has been widely applied in genome editing, synthetic biology, and transcriptional modulation in cells and animals. Toward more precise genomic modification and further expansion of the CRISPR/Cas9 system as a spatiotemporally controlled gene regulatory system, several approaches of conditional activation of Cas9 function using small molecules and light have recently been developed. These methods have led to improvements in the genome editing specificity of the CRISPR/Cas9 system and enabled its activation with temporal and spatial precision.
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