Journal
NATURE COMMUNICATIONS
Volume 10, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-09088-y
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Funding
- National Science Foundation (NSF) [NSF MCB 1803786]
- Physics Frontier Center for the Physics of Living Cells - NSF PHY [1430124]
- Beckman Institute Imaging Technology Group (University of Illinois)
- NIH [5R 24RR01744]
- Direct For Mathematical & Physical Scien
- Division Of Physics [1430124] Funding Source: National Science Foundation
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As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperaturejump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.
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