Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 12, Pages 4007-4010Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201600077
Keywords
affinity measurements; dissociation constants; high-throughput screenings; kinetics; protein-protein interactions
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Funding
- NIH [R01AI085583, R01CA170820]
- Ming Hsieh Institute for Research on Engineering-Medicine for Cancer
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There is great demand for high-throughput methods to characterize ligand affinity. By combining mRNA display with next-generation sequencing, we determined the kinetic on- and off-rates for over twenty thousand ligands without the need for synthesis or purification of individual members. Our results are reproducible and as accurate as those obtained with other methods of affinity measurement.
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