Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 55, Issue 30, Pages 8581-8584Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201602328
Keywords
cancer; circulating tumor cells; metabolism; microfluidics; picodroplets
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Funding
- Ministry of Education, Culture and Science [024.001.035]
- ERC
- CRO
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The number of circulating tumor cells (CTCs) in blood is strongly correlated with the progress of metastatic cancer. Current methods to detect CTCs are based on immunostaining or discrimination of physical properties. Herein, a label-free method is presented exploiting the abnormal metabolic behavior of cancer cells. A single-cell analysis technique is used to measure the secretion of acid from individual living tumor cells compartmentalized in microfluidically prepared, monodisperse, picoliter (pL) droplets. As few as 10 tumor cells can be detected in a background of 200 000 white blood cells and proof-of-concept data is shown on the detection of CTCs in the blood of metastatic patients.
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