Journal
VIRUSES-BASEL
Volume 11, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/v11020161
Keywords
Mice; Vero; Huh7; Monocyte Derived Macrophages; Virus isolation; Semen; Breast milk; Ebola virus
Categories
Funding
- NIAID Division of Intramural Research
- NIAID Division of Clinical Research
- Battelle Memorial Institute [HHSN272200700016I]
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Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectious virus. Virus isolation, typically using cell culture or animal models, can serve as a tool to determine the infectivity of patient samples. However, the sensitivity of these methods has not been assessed for the Ebola virus isolate, Makona. Described here is an efficiency comparison of Ebola virus Makona isolation using Vero E6, Huh-7, monocyte-derived macrophage cells, and suckling laboratory mice. Isolation sensitivity was similar in all methods tested. Laboratory mice and Huh-7 cells were less affected by toxicity from breast milk than Vero E6 and MDM cells. However, the advantages associated with isolation in Huh-7 cells over laboratory mice, including cost effectiveness, sample volume preservation, and a reduction in animal use, make Huh-7 cells the preferred substrate tested for Ebola virus Makona isolation.
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