Journal
VIRUS RESEARCH
Volume 261, Issue -, Pages 65-71Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2018.12.015
Keywords
Dengue virus; NS3; ALIX; AIP1; ESCRT; PDCD6IP
Categories
Funding
- Mahidol University
- Thailand Research Fund [IRN60W0002, BRG6080006]
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The endosomal sorting complexes required for transport (ESCRT) pathway accessory protein apoptosis linked gene-2-interacting protein X (ALIX) has been shown to be upregulated during dengue virus (DENV) replication. Yeast-two-hybrid screens have additionally shown that ALIX interacts with DENV NS3 protein, but evaluation of the interaction through a replicon assay failed to show a functional significance to the interaction. In this study the interaction between DENV NS3 and ALIX was investigated by co-immunoprecipitation, and functional significance assessed by investigation of DENV production in ALIX expression regulated cells. The results showed that ALIX both interacted and co-localized with DENV NS3 protein and that upregulation of ALIX resulted in a significantly increased viral titer, while either siRNA or CRISPR-Cas9 mediated down regulation of ALIX significantly reduced viral production, without affecting relative DENV genome levels. These results are consistent with ALIX playing a significant role in the DENV replication cycle either during late infection or at viral egress.
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