Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 208, Issue -, Pages 34-43Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2018.11.018
Keywords
African swine fever virus; Heterologous DNA-protein immunization; Plasmid-expressed antigen; Recombinant protein; Immune response; Immunopathology
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Funding
- U.S. Department of Homeland Security [DHS-2010-ST-061-AG0001]
- State of Kansas National Bio and Agro-Defense Facility (NBAF)
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African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-gamma ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.
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