Journal
VACCINE
Volume 37, Issue 17, Pages 2348-2355Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2019.03.027
Keywords
HIV-1 infection; Children; B cells; Homing; Chemokine receptors; HBV vaccine
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Funding
- Swedish Medical Research Council [2016-01165]
- Swedish International Development Agency [51080091]
- Swedish Research Council [2016-01165] Funding Source: Swedish Research Council
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Background: Successful vaccinations rely on antibody responses. Chemokine receptors play an important role in B cell homing to differentiation niches. We assessed CXCR4, CXCR5 and CCR6 expression on B cells during HIV-1 infection and relate it to antibody responses against a HBV vaccine. Methods: Blood was obtained from 54 healthy controls and 38 ART-treated HIV-1 infected children, aviremic (n = 25) or viremic (n = 13). Frequency of naive and memory B cell subsets was studied by immunostaining. Homing capacity of blood B cells to lymphoid and inflamed tissues was evaluated through CXCR4, CXCR5 and CCR6 expression. Plasma CXCL12 and CXCL13 levels and antibody titers to HBV antigen were determined by ELISA. Results: The frequency of naive and resting memory (RM) B cells in ART treated children was comparable to control subjects. Profound defects in the homing phenotypes of naive and memory B cells were identified, with lower CXCR4 and CXCR5 expression. Increased CXCL13 levels were observed in infected children, inversely correlating to CXCR5 expressing B cell subpopulations. Antibody titers to HBV vaccine correlated with frequency of resting and switched memory B cells in HIV-1 infected children. Conclusions: Homing defects of B cells to germinal center may underlie impaired vaccine responses during HIV-1 infection. (C) 2019 The Authors. Published by Elsevier Ltd.
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