Journal
TRENDS IN BIOTECHNOLOGY
Volume 37, Issue 8, Pages 870-881Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2019.01.009
Keywords
-
Categories
Funding
- 'KRIBB Research Initiative Program' by the Ministry of Science and ICT [NRF-2016M3A9B6903343]
- 'Bio & Medical Technology Development Program' by the Ministry of Science and ICT [NRF-2016M3A9B6903343]
- 'R&D Convergence Program' of the National Research Council of Science Technology [CAP-15-03-KRIBB]
Ask authors/readers for more resources
CRISPR technology is a two-component gene editing system in which the effector protein induces genetic alterations with the aid of a gene targeting guide RNA. Guide RNA can be produced through chemical synthesis, in vitro transcription, or intracellular transcription. Guide RNAs can be engineered to have chemical modifications, alterations in the spacer length, sequence modifications, fusion of RNA or DNA components, and incorporation of deoxynucleotides. Engineered guide RNA can improve genome editing efficiency and target specificity, regulation of biological toxicity, sensitive and specific molecular imaging, multiplexing, and editing flexibility. Therefore, engineered guide RNA will enable more specific, efficient, and safe gene editing, ultimately improving the clinical benefits of gene therapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available