Protective effect of Luteolin against methyl methanesulfonate-induced toxicity

The third instar larvae of transgenic Drosophila were allowed to feed on the diet having 10 mu M of MMS alone and along with 10, 20 and 40 mu M of Luteolin for 24 hrs. After the completion of 24 hrs, the larvae were subjected to ONPG assay, X-gal staining, trypan blue exclusion test, oxidative stress markers, apoptosis and comet assays. A dose-dependent increase in GSH content and decrease in the beta-galactosidase activity, tissue damage, GST activity, LPO, PCC, Caspase-9/3 activities, apoptotic index, and DNA damage was observed in the larvae exposed to 10, 20 and 40 mu M of Luteolin along with 10 mu M of MMS.

Automated summary

The study found that in transgenic Drosophila larvae, when combined with MMS, Luteolin resulted in a dose-dependent increase in GSH content and decrease in tissue damage and apoptosis.