Journal
THERIOGENOLOGY
Volume 126, Issue -, Pages 303-309Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2018.12.025
Keywords
Spermatozoa; Sperm capacitation; Proteomics; TMT; Buffalo
Categories
Funding
- Natural Science Foundation of Guangxi [2016GXNSFAA380225]
- National Natural Sciences Foundation of China [31160421]
- Guangxi Graduate Innovative Program [YCSW2018027]
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To investigate the proteomic profiling in buffalo spermatozoa before and after capacitation, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with Tandem Mass Tag (TMT) labeling strategy was applied. As a result, 1461 proteins were identified, 93 of them were found to be differentially expressed (>1.5-fold), including 52 up-regulated proteins and 41 down-regulated proteins during sperm capacitation. 88 out of 93 proteins were annotated and classified. Gene ontology (GO) analysis revealed that most of the differently expressed proteins (DEPs) were involved in the Biological Process of transport, cytoskeleton organization, sexual reproduction, and spermatogenesis. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that DEPs were mainly involved in the pathways of metabolic pathways, PPAR signaling pathway, and oxidative phosphorylation. Western blot (WB) assay confirmed the expressional variation of VAMP4 and APOC3 proteins. Our date provided a foundation for studying the changes in protein expression during sperm capacitation, which contributing to identifying marker proteins that may be associated with sperm capacitation. (C) 2018 Elsevier Inc. All rights reserved.
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