Journal
TALANTA
Volume 194, Issue -, Pages 903-909Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2018.11.014
Keywords
Surface enhanced Raman spectroscopy (SERS); Ag film@nano-Au substrate; Centrifugal microfluidic chip; Blood separation; Rapid diagnostics
Categories
Funding
- National Natural Science Foundation of China [21375156]
- National High Technology Research and Development Program of China (Ministry of Science and Technology 863 Plan) [2015AA021104]
- Fundamental Research Funds for the Central Universities
- Key Project (Fund for Brain Science) [10611CDJXZ238826]
- Chongqing University in China [02100011044104]
- State Key Laboratory of Transducer Technology, China [SKT1705]
- Major Theme Project of Chongqing Artificial Intelligence Technology Innovation [cstc2017rgan-zdyfX0019]
- Fundamental Research Funds for the Central Universities [106112017CDJPT120002]
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A microfluidic SERS chip integrated with blood separation and in-situ detection was designed and fabricated for the rapid detection of clinical blood samples. Each functional unit in the microfluidic SERS chip consist of separation-decantation cavity based on centrifugal separation principle, mixing channels and SERS detection chamber built with integrated nano-Au on Ag film microstructure. The serum creatinine was selected as a typical sample to demonstrate the capability of microfluidic SERS chip. It was found that the creatinine SERS characteristic peaks at 678 cm(-1) can be effectively identified and the detection limit could be as low as 4.42 x 10(-3) mu mol mL(-1) in water. The blood samples were also tested in microfluidic SERS chip. The whole separation and test process could be completed within 2 min, which is a significant improvement in the field of creatinine detection. The whole blood of six cases clinical blood samples were also tested, and the results were consistent with the enzymatic results. The developed microfluidic SERS chip has advantages including reduction of the required quantity of blood sample, reusable and easy to operate. It is expected to provide a new method for rapid diagnostics.
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