Journal
TALANTA
Volume 194, Issue -, Pages 266-272Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2018.10.035
Keywords
17 beta-Estradiol; Graphene; Electrochemical aptasensor; Signal cycling amplification
Categories
Funding
- National Natural Science Foundation of China (NSFC) [21777115, 21537003, 21377092]
- Science & Technology Commission of Shanghai Municipality [14DZ2261100]
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In the present work, a convenient signal-on electrochemical label-free aptasensor for 17 beta-estradiol (E2), a typical steroidal hormones endocrine disrupting chemicals, was proposed. 6-mercapto-1-hexanol (MCH) self-assembled monolayer (SAM) modified Au (MCH/Au) electrode was used as the substrate electrode. Graphene is used with bi-functions, not only to adsorb E2 binding aptamer, serving as the recognition element to E2, but also to be assembled onto MCH/Au electrode when sensing E2, to controllably turn on the electron transfer (eT), and further indicate the signal to E2 concentration. With the synergistic effect of DNase I enzyme, highly sensitive detection of E2 was achieved at this aptasensing system, with a linear range from 0.07 to 10 pM and a detection limit of 50 fM. An outstanding selectivity towards E2 was proven for the sensing system by simultaneously detecting 100-fold potential co-existing interferences. The stability and reproducibility were also evaluated to be satisfactory. Spiked real water analysis further indicated its reliability and potential in practical environmental monitoring.
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