Journal
TALANTA
Volume 194, Issue -, Pages 308-313Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2018.10.006
Keywords
Ratiometric fluorescence; Lipid droplets-targeting; Endogenous hypochlorous acid; Cell image
Categories
Funding
- Natural Science Foundation of Shandong Province [ZR2018MB042]
- National Natural Science Foundation of China [31871407]
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Lipid droplets were found to be involved in many organism activities. Here, a lipid droplets-targeted near infrared fluorescence probe (named XHZ) for ratiometric detection of endogenous hypochlorous acid/hypochlorite (HClO/ClO-) in living cells was developed, which was constructed by a coumarin moiety and a malononitrile derivative. XHZ could detect HClO/ClO- with high selectivity and sensitivity in a ratiometric manner based on FRET (Forster Resonance Energy Transfer) mechanism. The two well-resolved emission (470/672 nm) bands could ensure accurate detection of HClO/ClO- in vitro as well as in vivo. XHZ was successfully used for ratiometric fluorescence imaging of exogenous and endogenous HClO/ClO- in RAW264.7 cells. A good linear relationship between the fluorescence intensity ratios of the two emissions and HClO/ClO- concentrations from 0 to 40 mu m was obtained. Importantly, XHZ could localize mainly in lipid droplets of RAW264.7 cells. To the best of our knowledge, XHZ is the first lipid droplets-targeted ratiometric fluorescence probe for HClO/ClO-.
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