Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 283, Issue -, Pages 563-570Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.12.076
Keywords
Diclofenac; Immunochromatographic assay (ICA); Surface-enhanced Raman scattering (SERS); SERS-ICA; Ag@SiO2 core-shell nanoparticles
Funding
- National Natural Science Foundation of China (NSFC) [21075087, 21175097, 81473166, 31772053]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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In the last years, surface-enhanced Raman scattering based immunochromatographic assay (SERS-ICA) has been getting great attention and been applied for the detection of different target analytes. In this study, a novel SERS-ICA using Ag-MBA@SiO2-Ab as an immunoprobe has been developed for rapid, quantitative and ultrasensitive detection of a pharmaceutical diclofenac (DCF) residue in water samples. Ag, Ag@SiO2 and Ag-MBA@SiO2 nanoparticles (NPs) were synthesized and characterized. The immunoprobe was prepared by sandwiching the Raman reporter mercaptobenzoic acid (MBA) between the core-shell layers and immobilizing the monoclonal antibody (mAb) against DCF on the surface of the Ag@SiO2 NPs. Under optimal conditions, the sensitivity and limit of detection (LOD) of the SERS-ICA for DCF were found to be 9 pg mL(-1) and 0.07 pg mL(-1), respectively. The detection of the SERS-ICA for DCF was completed within 15 min. Ag-MBA@SiO2-Ab was stable within four months without significant loss of SERS intensity. The key to success of the SERS-ICA was the employment of the Ag@SiO2 NPs as the SERS substrate, which possessed gorgeous SERS enhancement capability, predominant biocompatibility and excellent stability. This SERS-ICA is potential as an ultrasensitive analytical method in point-of-care testing, clinical diagnosis, food inspecting and environmental monitoring.
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