Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 283, Issue -, Pages 207-214Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.12.026
Keywords
Immunoassay; Fluorescence; Composite; Imidacloprid; Alkaline phosphatase
Funding
- National Key Research and Development Program of China [2016YFC0207300]
- National Nature Science Foundation of China [61831011, 61503148, 61520106003, 21806051]
- Science and Technology Development Program of Jilin Province [2017052016JH]
- Program for JLU Science and Technology Innovative Research Team [JLUSTIRT 2017TD-07]
- Scientific and Technological 13th Five-Year Plan Project of Jilin Provincial Department of Education [JJKH20190111KJ]
- China Postdoctoral Science Foundation [2018M631862, 2017M621199]
- National Postdoctoral Program for Innovative Talents [BX201700096]
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The fabrication of a convenient fluorescence-based immunoassay (FIA) for specific antigen attract increasing concern but remains a considerable challenge, especially the laborious synthesis of nanomaterials-antibody conjugates. Herein, we circumvent this drawback by introducing a specific fluorescence signal response procedure to commercially available alkaline phosphatase (ALP)-labeled conventional immunoassay for imidacloprid detection. In this FIA protocol, gold nanoclusters (AuNCs) can anchor on the surface of two-dimensional cobalt oxyhydroxide (CoOOH) nanoflakes to form nanocomposite, resulting in remarkable decrease of fluorescence intensity. The quenching effects can be effectively reversed by introducing ascorbic acid that can trigger the decomposition of CoOOH nanoflakes. Notably, the corresponding fluorescence response induced by ascorbic acid was related to ALP activities labeled on antibody. After competitive immunoreaction, the ALP-labeled antibodies were bounded to immobilized antigen, which can regulate the fluorescence change. Utilizing fluorescence switching of system, the 50% inhibition concentration (IC50) value of FIA toward imidacloprid was obtained at 1.3 ng mL(-1) which was 60-fold-of-magnitude more sensitive than that of conventional ELISA (86.4 ng mL(-1)). This FIA protocol not only develops new prospects for pesticide detection, but also opens up potent strategy of fluorogenic immunoassay.
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