Journal
SENSORS
Volume 19, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/s19030692
Keywords
magnetic microparticles; immunosensor; tumor necrosis factor-alpha; chronoamperometry; saliva analysis; heart failure
Funding
- Ministry of Tunisian Higher Education and Scientific Re-search
- HEARTEN [643694]
- KardiaTool [768686]
- University of Sousse
Ask authors/readers for more resources
Tumor necrosis factor-alpha (TNF-alpha) is a biomarker of inflammation that occurs in patients suffering from heart failure (HF). Saliva can be sampled in a non-invasive way, and it is currently gaining importance as matrix alternative to blood in diagnostic and therapy monitoring. This work presents the development of an immunosensor array based on eight screen-printed gold electrodes to detect TNF-alpha in saliva samples. Two different functionalization strategies of electrodes were compared. In the first, anti-TNF-alpha antibodies were chemically bonded onto the electrode by functionalization with 4-carboxymethylaniline. The other functionalization procedure involved the binding of antibodies onto polymer-coated magnetic microparticles, which were then deposited onto the electrode by pulsed chronoamperometry. Finally, the chronoamperometry technique was applied to characterize the modified SPEAu. The use of a secondary antibody anti-TNF-alpha (Ab-TNF-alpha-HRP) labelled with horseradish peroxidase (HRP, 2 mu g.mL(-1)) was investigated using tetramethylbenzidine (TMB, pH = 3.75) as electrochemical substrate containing 0.2 mM of H2O2. A sandwich-type detection strategy with a secondary antibody anti-TNF-alpha provided chronoamperometric analyses in 10 s for each sample. Linearity, precision, limit of detection, and selectivity of devices were investigated. Interferences were evaluated by analyzing solutions containing other cytokine produced during the acute stage of inflammation. The immunosensor showed good performance within the clinically relevant concentration range, with a precision of 8%, and a limit of detection of 0.3 pg/mL. Therefore, it may represent a promising tool for monitoring HF in a non-invasive way.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available