Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 649, Issue -, Pages 686-694Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2018.08.318
Keywords
Biological nitrogen fixation; Molybdenum; Rice; 16S rRNA gene; DNA-SIP; Cyanobacteria
Categories
Funding
- Special Project on the Basis of the National Science and Technology of China, China [2015FY110700]
- Natural Science Foundation of China, China [NSFC-40871146, NSFC-41501273]
- Knowledge Innovation Program of the Chinese Academy of Sciences, China [KZCX2-EW-409]
- Technology Supporting Project of Jiangsu Province, China [BE2013451]
Ask authors/readers for more resources
Molybdenum (Mo) deficiency in the farmland of China may limit biological nitrogen fixation (BNF), however, the impact of Mo application on BNF capacities and diazotrophic communities in rice-soil systems is unclear. In this experiment, treatments in a 6.7 atom% N-15(2)-labelling field-based growth chamber for 74 days and treatments in a 99 a tom% N-15(2)-labelling microcosm experiment for 40 days combined with 16S rRNA gene sequencing and DNA-stable isotope probing (SIP) were used to investigate the impacts of Mo application on BNF and diazotrophic communities. Our results showed that under the condition that no nitrogen (N) fertilizer was applied. Mo application (500g sodium molybdate ha(-1)) significantly increased N-2 fixation in a rice-Inceptisol system, from 22.3 to 53.1 kg N ha(-1). Mo application significantly increased the number of nifH gene copies and the relative abundance of cyanobacteria in both growth chamber and microcosm experiments. Among cyanobacteria, the relative abundances of the most abundant genera Leptolyngbya and Microcoleus were significantly increased by Mo application. N-15(2) -DNA-SIP further demonstrated that Leptolyngbya and Microcoleus incorporated N-15(2). Mo application greatly increased BNF in Mo-deficient paddy field (<= 0.068 mg kg(-1)) and stimulated the growth of cyanobacteria. These results indicated that Mo application in Mo-deficient paddy field could be a useful measure to increase soil N input under no N fertilization. (C) 2018 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available