Journal
SCIENCE CHINA-LIFE SCIENCES
Volume 62, Issue 5, Pages 619-632Publisher
SCIENCE PRESS
DOI: 10.1007/s11427-018-9500-7
Keywords
protein-protein interactions; in vivo; techniques; RET; PCA; co-localization
Categories
Funding
- National Natural Science Foundation of China [31530084, 31761133009]
- Programme of Introducing Talents of Discipline to Universities [B13007]
Ask authors/readers for more resources
Detecting protein-protein interactions (PPIs) provides fundamental information for understanding biochemical processes such as the transduction of signals from one cellular location to another; however, traditional biochemical techniques cannot provide sufficient spatio-temporal information to elucidate these molecular interactions in living cells. Over the past decade, several new techniques have enabled the identification and characterization of PPIs. In this review, we summarize three main techniques for detecting PPIs in vivo, focusing on their basic principles and applications in biological studies. We place a special emphasis on their advantages and limitations, and, in particular, we introduced some uncommon new techniques, such as single-molecule FRET (smFRET), FRET-fluorescence lifetime imaging microscopy (FRET-FLIM), cytoskeleton-based assay for protein-protein interaction (CAPPI) and single-molecule protein proximity index (smPPI), highlighting recent improvements to the established techniques. We hope that this review will provide a valuable reference to enable researchers to select the most appropriate technique for detecting PPIs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available