Journal
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL
Volume 52, Issue -, Pages -Publisher
SOC BRASILEIRA MEDICINA TROPICAL
DOI: 10.1590/0037-8682-0300-2018
Keywords
Sepsis; C. elegans; Chemotaxis assay; C. elegans Sepsis Detection Assay (CESDA)
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Funding
- Universiti Kebangsaan Malaysia [GGPM-2013-102]
- Ministry of Higher Education, Malaysia [FRGS/1/2014/SKK01/UKM/03/3]
- Ministry of Science, Technology & Innovation (MOSTI) [SA1212001]
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Introduction: The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). Methods: C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. Results: The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). Conclusions: CESDA could be further explored for sepsis diagnosis.
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