Journal
ANDROLOGIA
Volume 48, Issue 9, Pages 961-966Publisher
WILEY
DOI: 10.1111/and.12538
Keywords
Caffeine; cryopreservation; mitochondria; sperm motility; spermatozoa
Categories
Funding
- Androscience, High Complexity Clinical and Research Andrology Laboratory, Sao Paulo, SP, Brazil
- Capes-PROEX
- National Council for Scientific and Technological Development (CNPq) [301373/2013]
Ask authors/readers for more resources
This study aimed to evaluate the effects of caffeine supplementation in post-cryopreservation human semen over different incubation periods. After collection by masturbation, 17 semen samples were analysed according to World Health Organization criteria, processed and cryopreserved with TEST-yolk buffer (1:1) in liquid nitrogen. After a thawing protocol, samples were incubated with 2mm of caffeine for 0, 5, 15, 30 or 60min, followed by analysis of motility and mitochondrial activity using 3,3-diaminobenzidine (DAB). Mean variance analysis was performed, and P<0.05 was the adopted significance threshold. Samples incubated for 15min showed increased progressive motility compared to other periods of incubation, as well as a reduced percentage of immotile spermatozoa (P<0.05). In samples incubated for 5min, increased mitochondrial activity above 50% was observed (DABI and DABII). Although cryosurvival rates were low after the cryopreservation process, incubation with caffeine was associated with an increase in sperm motility, particularly 15-min incubation, suggesting that incubation with caffeine can be an important tool in patients with worsening seminal quality undergoing infertility treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available