Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 49, Issue 4, Pages 375-383Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2019.1573195
Keywords
Biopolymers; bioremediation; endocrine disrupter; immobilization; laccase extract
Categories
Funding
- CAPES/Brazil
Ask authors/readers for more resources
Laccase extract (LE) from Pycnoporus sanguineus was immobilized on calcium and copper alginate-chitosan beads and applied for the removal of 17 alpha-ethinylestradiol (EE2). Effects of immobilization conditions such as: sodium alginate (SA) concentration; LE/SA ratio and chitosan/ion (Ca+2 or Cu+2) ratio on the immobilization yield were investigated. Immobilized LE on Ca-beads and Cu-beads was then used to degrade an EE2 solution. The optimal conditions for LE immobilization on Ca-beads were: 1.5% (w/v) SA, 1:5 (v/v) LE/SA and 3:7 (v/v) chitosan/ion (Ca+2). The optimal conditions for immobilization on Cu-beads were 2.0% (w/v) SA, 0.5:5 (v/v) LE/SA and 3:7 (v/v) chitosan/ion (Cu+2). The best result was obtained for immobilized LE on Ca-beads in buffer-absent medium. Furthermore, the immobilized enzyme was reused in five cycles for EE2 removal. The formation of EE2 dimers by LE treatment has been demonstrated by electrospray ionization coupled to time of flight mass spectrometer (ESI-TOF-MS). The results evidenced that immobilized LE in alginate-chitosan-divalent cation bead is an effective alternative for EE2 removal.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available