Journal
ANALYTICAL SCIENCES
Volume 32, Issue 3, Pages 323-328Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.32.323
Keywords
Graphene oxide; electrochemical immunosensor; human immunoglobulin G; biomarker; screen-printed carbon electrode
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Funding
- Thailand Research Fund [MRG5680058]
- Research, Development and Engineering (RD&E) fund through the National Nanotechnology Center (NANOTEC), The National Science and Technology Development Agency (NSTDA), Thailand [P-11-00992]
- Development and Promotion of Science and Technology talents project through the Institute for the Promotion of Teaching Science and Technology (IPST), the National Research University Project under Thailand's Office of the Higher Education Commission [DPST 034/2013]
- Chiang Mai University
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In this work, a cost-effective and simple-to-prepare label-free electrochemical immunosensor was, for the first time, fabricated by modifying high-quality graphene oxide (GPO) onto a screen-printed carbon electrode (SPCE). The anti-IgG antibody was then covalently immobilized to the carboxylic group anchoring on the surface of GPO particles. Under the optimized condition, our newly developed immunosensor selectively bound to human immunoglobulin G (IgG), a model biomarker, with high sensitivity at a limit of detection of 1.99 ng mL(-1), potentially sensitive enough for IgG detection at the pathophysiological level, and had a linear range of 2.5 to 100 ng mL(-1). The proposed immunosensor also exhibited high reproducibility and regenerability, resulting in no significant change in electrochemical signals from different replicates of the electrode, and a robust electrochemical current after being subjected to alkaline base washing with several cycles. To this end, our immunosensor demonstrates ability as a promising diagnostic tool for clinical assessment.
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