Journal
ANALYTICAL METHODS
Volume 8, Issue 15, Pages 3055-3060Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ay03295d
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Funding
- SAFEMEAT [PON01_01409]
- ONEV [PONa3_00354]
- Regione Puglia NABIDIT - NANOBIOTECNOLOGIE e SVILUPPO PER TERAPIE INNOVATIVE Project funds [F31D08000050007]
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Foodborne illnesses caused by the ingestion of foods contaminated with pathogens and/or their toxins are still one of the major public health threats worldwide. Disposable devices, allowing the on-site, early and multiplexed quantitative detection of pathogenic bacteria are therefore highly sought. Herein, we report biochips that are able to quantitatively detect two of the most common food-associated pathogens, namely Listeria monocytogenes and Staphylococcus aureus from the suspensions of bacteria stationary-phase broth culture. With a detection limit as low as 5.00 CFU ml(-1) for L. monocytogenes and 1.26 CFU ml(-1) for S. aureus, our platform may be a promising point-of-care device not only for clinical and food diagnostics but also for biosecurity purposes.
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