Journal
PLANT GROWTH REGULATION
Volume 88, Issue 1, Pages 61-71Publisher
SPRINGER
DOI: 10.1007/s10725-019-00488-0
Keywords
Polyamines; Tomato; Arginine decarboxylase; Fruit-specific promoter; Transgenic plants; Fruit quality
Categories
Funding
- Department of Biotechnology (Govt. of India), New Delhi [BT/PR/2990/Agr/16/232/2002, BT/PR8657/PBD/16/738/2007]
- University Grants Commission for Special Assistance Programme (DRS-III)
- Department of Science and Technology (DST) for FIST (Level 2) programme
- DU-DST PURSE Phase II grant
- Council of Scientific and Industrial Research, New Delhi
- Department of Biotechnology
Ask authors/readers for more resources
Over-ripening is the key contributor to post-harvest loss of perishable fruits and is also one of the greatest threats to the growers. Delayed ripening of tomato fruits has been suggested as a possible solution to such post-harvest losses. To overcome post-harvest losses, we engineered tomato fruits to accumulate polyamines (PAs) by over-expression of arginine decarboxylase (ADC) gene of PA biosynthetic pathway. The AsADC transgenic fruits contained elevated levels of putrescine, spermidine and spermine content in comparison to the fruits from untransformed plants. In addition, the expression of several ripening-associated genes was also altered. The transgenic fruits displayed a better shelf-life due to decrease in ethylene levels and respiratory activity. The transgenic tomatoes also exhibited improved key processing features along with the elevated levels of lycopene and vitaminC. Our study highlights that the fortification of PAs is a promising approach for the improvement of post-harvest fruit characteristics together with a high quality of produce.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available