Journal
ANALYTICAL METHODS
Volume 8, Issue 15, Pages 3069-3074Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ay03396a
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Supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels (SIL-g-(N) GAs) were used as a novel electrochemical DNA biosensor for the direct detection of the breast cancer-related BRCA1 gene. The sensor performance was monitored by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). This detection was done without any kind of enzyme, label, or sophisticated equipment. The sensor was found to be stable, reproducible, ultraselective and ultrasensitive as a limit of detection (LOD) of 2 pM was obtained by fitting the experimental data with linear equations. According to the results, this biosensor was able to perform DNA hybridization and DNA sequencing simultaneously.
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