Journal
ANALYTICAL METHODS
Volume 8, Issue 40, Pages 7413-7419Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ay01705c
Keywords
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Funding
- National Natural Science Foundation of China [21575035]
- Foreign Science and Technology Cooperation Fund of Hubei province, China [2015BHE025]
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The sequence-specific recognition of SNPs is important for disease diagnosis and human gene therapy. In this work, a simple sensor based on electrochemical impedance spectroscopy (EIS) was developed for the highly sensitive and specific detection of G-G mismatches in dsDNA using a small molecule-modified gold electrode. After the electrochemical impedance sensor was incubated with G-G mismatched dsDNA, its charge-transfer resistance (R-ct) increased significantly. Meanwhile, only a small increase in R-ct values was observed for dsDNA with other base mismatches and for complementary dsDNA. Importantly, G-G mismatched dsDNA was selectively detected based on differences in charge transfer resistance (Delta R-ct) even in the presence of other DNA and ct-DNA. Under optimal experimental conditions, the established EIS sensor was used to quantify G-G mismatched dsDNA based on the linear relationship between Delta R-ct and the logarithm of the concentration of G-G mismatched dsDNA from 1 nM to 1 mu M with a detection limit of 0.3 nmol L-1. This sensing strategy is a potential alternative tool for measuring G-G mismatched DNA for the diagnosis and early clinical detection of genetic diseases.
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