Journal
ANALYTICAL METHODS
Volume 8, Issue 25, Pages 5123-5128Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ay01296e
Keywords
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Funding
- Ministry of Education, Youth and Sports of the Czech Republic [LQ1601]
- Internal Grant Agency of Mendel University in Brno [IP_33_2015]
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Early detection of antibiotic-resistant bacteria causing inflammation in patients is a key for an appropriate and timely treatment. Classical microbiological cultivation methods are time-consuming and therefore it is necessary to discover a quick and effective method. The method used in this study is based on a sandwich immunoreaction of bacteria with IgG immobilized on streptavidin-coated polystyrene superparamagnetic particles and MRSA-specific antibody immobilized on gold non-magnetic nanoparticles labeled with oligonucleotides, which were detected by square wave voltammetry in combination with the adsorptive transfer technique. The assay is suitable for selective detection of methicillin-resistant Staphylococcus aureus in samples even at a concentration of 2 x 10(4) CFU mL(-1). The method can be flexibly modified for any other bacteria, depending on the antibodies used.
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