Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 19, Pages 9746-9752Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b02646
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Funding
- National Natural Science Foundation of China [21005068, 21475114]
- Hunan Provincial Natural Science Foundation of China [13JJ6039, 2015JJ6104]
- China Postdoctoral Science Foundation [2014M552140, 2015T80876]
- State Key Laboratory of Chemo/Biosensing and Chemometrics Foundation [2015007]
- Scientific Research Fund of Hunan Provincial Education Department [15K125]
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Glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) are small-molecular biothiols that play key roles in various biological systems. Among these biothiols, GSH is the most abundant intracellular thiol. Until now, a small number of the near infrared (NIR) fluorescent probes have been designed for the detection of GSH. Unfortunately, most of these NIR probes are based on cyanine dyes, which generally suffer low fluorescence quantum yield (Phi < 0.25), which are not suitable for bioimaging. In addition, some probes are difficult to effectively distinguish GSH from Cys and Hcy. In this work, an NIR fluorescent probe with high fluorescence quantum yield is developed by introducing a rigid coplanar structure such as rhodamine dyes, and the NIR probe (CyR) with spirolactam structure is first synthesized and used to recognize GSH. The characteristics of this NIR. probe are as follows: (1) probe CyR exhibits high fluorescence quantum yield (Phi = 0.43) after the addition of GSH and high sensitivity toward GSH with 75-fold fluorescence enhancement. (2) The probe is highly selective, which will not interfere with the other biological thiols (Cys, Hcy) and amino acids. (3) A possible reaction mechanism of the NIR probe CyR and GSH (Cys, Hcy) can be proposed and proved by H-1 NMR, C-13 NMR, and MS (mass spectra). (4) The NIR probe displays selective detection of GSH in biological samples such as living cells and tissues.
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