Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 7, Pages 3872-3878Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b00023
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Funding
- National Basic Research Program of China [2015CB856503]
- NSFC [31571011, 81301311, 81220108015, 51222303]
- PCSIRT [IRT13023]
- Science and Technology Project of Tianjin of China [15JCYBJC29800]
- Singapore National Research Foundation [R-279-000-111-281]
- Singapore-MIT Alliance for Research and Technology (SMART) Innovation Grant [R279-000-378-592]
- Research Grants Council of Hong Kong [HKUST/CRF/10, N_HKUST620/11]
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Fluorescent light-up probes with aggregation-induced emission (AIE) characteristics have recently attracted great research interest due to their intelligent fluorescence activation mechanism and excellent photobleaching resistance. In this work, we report a new, simple, and generic strategy to design and prepare highly sensitive AIE fluorescent light-up bioprobe through facile incorporation of a self-assembling peptide sequence GFFY between the recognition element and the AIE luminogen (AIEgen). After the bioprobes respond to the targets, the peptide GFFY is capable of inducing the ordered self-assembly of AIEgens, yielding close and tight intermolecular steric interactions to restrict the intramolecular motions of AIEgen for excellent signal output. Using two proof-of concepts, we have demonstrated that self-assembling peptide incorporating ALE light-up probes show much higher sensitivity in sensing the corresponding targets in both solutions and cancer cells as compared to those without GFFY induced self-assembly. Taking the probe TPE-GFFYK(DVEDEE-Ac), for example, a detection limit as low as 0.54 pM can be achieved for TPE-GFFYK(DVEDEE-Ac) in caspase-3 detection, which is much lower than that of TPE-K(DVED-Ac) (3.50 pM). This study may inspire new insights into the design of advanced fluorescent molecular probes.
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