Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 12, Pages 6615-6620Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b01738
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Funding
- National Creative Research Initiative program of the National Research Foundation of Korea (NRF) - Korean government (MSIP) [2012R1A3A2048814]
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2012R1A2A1A03670456]
- National Research Council of Science and Technology through the Degree & Research Center program [DRC-14-1-KBSI]
- National Research Foundation of Korea [2012R1A3A2048814, 2012R1A2A1A03670456] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Hypochlorite (OCl-) plays a key role in the immune system and is involved in various diseases. Accordingly, direct detection of endogenous OCl- at the subcellular level is important for understanding inflammation and cellular apoptosis. In the current study, a two-photon fluorescent off/on probe (PNIS) bearing imidazoline-2-thione as an OCl- recognition unit and triphenylphosphine (TPP) as a mitochondrial-targeting group was synthesized and examined for its ability to image mitochondrial OCl- in situ. This probe, based on the specific reaction between imidazoline-2-thione and OCl-, displayed a selective fluorescent off/on response to OCl- with the various reactive oxygen species in a physiological medium. PNIS was successfully applied to image of endogenously produced mitochondrial OCl- in live RAW 264.7 cells-via two-photon microscopy.
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