Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 7, Pages 4114-4121Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b00449
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Funding
- National Natural Science Foundation of China [21435001, 21275079, 81227901, 61501462]
- National Basic Research Program of China [2011CB707703]
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Reliable long-term in vivo tracking of stem cells is of great importance in stem cell-based therapy and research. Fluorescence imaging with in situ excitation has significant autofluorescence background, which results in poor signal-to-noise ratio (SNR). Here we report TAT penetrating peptide-bioconjugated long persistent luminescence nanoparticles (LPLNP-TAT) for long-term tracking of adipose-derived stem cells (ASC) without constant external excitation. LPLNP-TAT exhibits near-infrared emitting, red light renewable capability, and superior in vivo imaging depth and SNR compared with conventional organic dye and quantum dots. Our findings show that LPLNP-TAT can successfully label ASC without impairing their proliferation and differentiation and can effectively track ASC in skin-regeneration and tumor-homing models. We believe that LPLNP-TAT represents a new generation of cell tracking probes and will have broad application in diagnosis and therapy.
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