Journal
ANALYTICAL CHEMISTRY
Volume 88, Issue 10, Pages 5046-5049Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b01514
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Funding
- National Basic Research Program of China (973 Program) [2013CB733700]
- National Natural Science Foundation of China [21421004, 21327807]
- Chang Jiang Scholars Program
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Aerolysin has been used as a biological nanopore for studying peptides, proteins, and oligosaccharides in the past two decades. Here, we report that wild-type aerolysin could be utilized for polynucleotide analysis. Driven a short polynucleotide of four nucleotides length through aerolysin occludes nearly 50% amplitude of the open pore current. Furthermore, the result of total internal reflection fluorescence measurement provides direct evidence for the driven translocation of single polynucleotide through aerolysin.
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