Journal
ONCOLOGY REPORTS
Volume 41, Issue 4, Pages 2431-2439Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2019.7027
Keywords
long non-coding RNAs; AFAP1 antisense RNA1; cervical cancer; prognosis; migration and invasion
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Funding
- National Natural Science Foundation of China [81572787, 81672683, 81672993, 81672688, 81702907, 81772901, 81772928, 81803025, 81872278]
- Overseas Expertise Introduction Project for Discipline Innovation (111 Project) [111-2-12]
- Natural Science Foundation of Hunan Province [2016JC2035, 2017SK2105, 2018JJ3704, 2018JJ3815, 2018SK21210, 2018SK21211]
- Foundation from the Changsha Science and Technology Board [kq1706045]
- Chinese Anti-Cancer Association
- Fundamental Research Funds for the Central Universities of Central South University [1053320171023]
- Special Fund of Clinical Medicine of Chinese Medical Association [17020280697]
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Although lncRNA AFAP1 antisense RNA1 (AFAP1-AS1) is considered an oncogenic lncRNA, little is known about the role of AFAP1-AS1 in cervical cancer. In the present study, we found that AFAP1-AS1 was elevated and hypomethylated in cervical cancer and was associated with a poor prognosis of patients with cervical cancer by analyzing the Cancer RNA-Seq Nexus (CRN), MethCH and UCSC XENA databases. Subsequently, we knocked AFAP1-AS1 expression down using siRNAs in cervical cancer cells. Wound healing experiments and matrigel invasion experiments revealed that the downregulation of AFAP1-AS1 suppressed the migration and invasion of cervical cancer cells. Furthermore, western blot analysis demonstrated that the antitumor effects induced by the silencing of AFAP1-AS1 were mainly mediated through the regulation of the Rho/Rac signaling pathway and epithelial-mesenchymal transition (EMT)-related genes. Taken together, the findings of the present study indicate that the expression level of AFAP1-AS1 may be involved in the development of cervical cancer. Thus, AFAP1-AS1 may be a novel prognostic biomarker and a potential therapeutic target for patients with cervical cancer.
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